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. 2021 Mar 19;9:620128. doi: 10.3389/fbioe.2021.620128

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Copyright © 2021 Wang, Jarman, Lin and Baker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of actomyosin activity and microtubule polymerization reduces collagen compaction. (A) Representative images (max intensity projections) of dimethylsulfoxide (DMSO) control, 25 μM blebbistatin-, and 50 ng ml^–1 nocodazole-treated sprouts cultured over 5 days with 250 nM sphingosine 1-phosphate (S1P) and 25 ng ml^–1 phorbol 12-myristate 13-acetate (PMA) within 3 mg ml^–1 collagen. (B) Representative images (individual z-slices) of fluorescently labeled collagen (intensity heat map) of conditions from panel (A). (C,D) Quantifications of sprout diameter and collagen compaction from conditions in panels (A,B). For sprout diameter: n ≥ 52 per condition and for collagen compaction: n ≥ 10 per condition. All data presented as mean ± SD; *indicates a statistically significant comparison to DMSO control with P < 0.05 (two-tailed Student’s t-test).