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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Toxicol Lett. 2021 Mar 13;344:58–68. doi: 10.1016/j.toxlet.2021.03.006

Figure 6. Luteolin-induced apoptosis, DNA damage, and cell cycle changes in the CYP1A1-expressing TK6 cells.

Figure 6.

Cells were treated with the indicated concentrations for 24 h. (A) Histogram shows the caspase 3/7 enzymatic activity comparing the CYP1A1-expressing cells to their EV controls. (B & C) Western blot shows the protein expression of CYP1A1, cleaved PARP-1, cleaved caspase 3, and γH2A.X in treated cells (B); all proteins were quantified and normalized to the amount of GAPDH (C). (D & E) Representative flow cytometry plots are shown from one experiment (D) and histogram is cell cycle changes comparing the CYP1A1-expressing cells to their EV controls. The data represent the mean ± SD from three independent experiments (A, C, & E). * indicates P < 0.05 comparing the CYP1A1-expressing cell line versus their corresponding EV controls at the same concentration.