Figure 4.

TR-Cubes MHT damage on patient CR-CSCs #21 and more specifically on qCR-CSCs #21. (a) Flow cytometry analysis of annexin V–FITC and PI staining was used to evaluate the cell viability following MHT treatment (f = 182 kHz reaching a fixed temperature of 45 °C for 90 min). Triplicates from the control nontreated cells and the cells exposed to MHT for 90 min were measured. The purple population in the dot plot represents live cells; green, the apoptotic fraction; and red, the dead PI-stained cells. (b) PKH^pos cell death estimation based on confocal microscope analysis using Sytox blue staining, specific for dead cells. Left panel, images acquired by confocal microscopy: the green signal represents PKH^pos cells, the blue signal represents dead cells, and colocalized green and blue represent dead PKH^pos cells. Right panel, cumulative representation of live (green) vs dead (blue) PKH^pos cell quantified from the acquired confocal images. At least 500 PKH^pos cells were counted per each time point in n = 3 independent experiments. The mean data are reported, and error bars represent SD. Scale bar of 50 μm.