. 2021 Apr 8;7(4):e06734. doi: 10.1016/j.heliyon.2021.e06734

Table 2.

Flavonoid content and antioxidant activity of the purified polyphenolic compounds of E. antisyphilitica by-products from the different wax-extraction processes.

Sample	Flavonoids		DPPH^•		ABTS^•+		Lipid oxidation		FRAP
	mE Quercetin (250 μg mL⁻¹)		(IC_50, μg mL⁻¹)		(IC_50, μg mL⁻¹)		(% Inh) 250 μg mL⁻¹		mE Gallic acid (250 μg mL⁻¹)
	E. H₂SO₄	E. C₆H₈O₇	E. H₂SO₄	E. C₆H₈O₇	E. H₂SO₄	E. C₆H₈O₇	E. H₂SO₄	E. C₆H₈O₇	E. H₂SO₄	E. C₆H₈O₇
León	51.80 ± 6.44^BC	50.85 ± 5.15^A	388.683 ± 7^B	211.267 ± 4.85^AB	68.16 ± 2.67^A	46.78 ± 2.04^A	45.91 ± 8.22^A	65.26 ± 6.16^A	56.45 ± 7.36^A	113.29 ± 6.83^B
SJ	43.71 ± 2.85^C	57.52 ± 25.16^A	319.2 ± 5.67^C	179.441 ± 7.92^B	66.96 ± 2.56^A	49.85 ± 3.05^A	45.75 ± 3.73^A	64.69 ± 1.23^A	50.172 ± 0.747^A	135.07 ± 6.01^A
SMYA	65.14 ± 1.42^AB	55.14^A	401.404 ± 6.45^B	189.61 ± 7.95^AB	69.01 ± 2.56^A	49.63 ± 0.54^A	43.37 ± 5.45^AB	70.31 ± 2.50^A	46.382 ± 1.635^A	123.40 ± 4.61^AB
LB	78.95 ± 10.81^A	64.66 ± 4.36^A	438.154 ± 11.53^A	239.43 ± 42.63^A	62.40 ± 4.65^A	48.55 ± 2.42^A	30.1 ± 4.29^B	60.71 ± 3.05^A	54.91 ± 3.09^A	124.12 ± 3.51^AB

The extractions were identified as E. H₂SO₄ (sulfuric acid) and E. C₆H₈O₇ (citric acid). The letters (A, B, C) show significant differences according to the comparison of means between the same extraction method (p = 0.05). % Inh represents the percentage of inhibition of linoleic acid oxidation.