(a) Arc-immunoreactivity in the IL showed a main effect of pre-stress treatment in male mice, and a near-significant effect in (b) female mice but in the opposite direction. (c, d) In the PL, no significant main effects or interactions of conditioning group and pre-stress treatment were found in either sex. (e) In the AI, there was a near-significant main effect of pre-stress treatment in male mice, (f) but no main effect of either conditioning group or pre-stress treatment and no interaction effect in female mice. n = 5-6 per group. S = saline, G = guanfacine, *p < 0.05