Table I.
Antibodies used for the western blot analysis.
| Primary antibody | Secondary antibody | |||||||
|---|---|---|---|---|---|---|---|---|
| Target | Supplier and cat. no. | Dil. | Inc. | Target | Supplier and cat. no. | Dil. | Inc. | |
| Bax | CS #5023 | 1:1,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| Bcl-2 | CS #3498 | 1:1,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| Noxa | Thermo 1-41000 | 1:1,000 | ON 4°C | Anti-mouse | Dako P0447 | 1:3,000 | 1 h 4°C | |
| TRAIL-R2 | CS #3696 | 1:1,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| cPARP | Thermo 436400 | 1:2000 | ON 4°C | Anti-mouse | Dako P0447 | 1:3,000 | 1 h 4°C | |
| LC3BI–II | CS #2775 | 1:1,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| Survivin | CS #2802 | 1:1,000 | ON 4°C | Anti-mouse | Dako P0447 | 1:3,000 | 1 h 4°C | |
| HSP90 | CS #4877 | 1:3,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| XIAP | CS #2045 | 1:3,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| Smad2 | sc-101153 | 1:1,000 | ON 4°C | Anti-mouse | Dako P0447 | 1:3,000 | 1 h 4°C | |
| Smad3 | sc-101154 | 1:1,000 | ON 4°C | Anti-mouse | Dako P0447 | 1:3,000 | 1 h 4°C | |
| γH2AX | CS #2577 | 1:1,000 | ON 4°C | Anti-rabbit | Dako P0448 | 1:3,000 | 1 h 4°C | |
| β-actin | sc-47778 | 1:10,000 | ON 4°C | Anti-mouse | Dako P0447 | 1:15,000 | 1 h 4°C | |
TRAIL-R2, TNF-related apoptosis-inducing ligand receptor 2; cPARP, cleaved poly(ADP-ribose) polymerase (PARP); HSP90, heat shock protein 90; XIAP, X-linked inhibitor of apoptosis protein; γH2AX; histone H2AX phosphorylation at serine 139; Dil., dilution; Inc, incubation; CS, Cell Signaling Technology, Inc.; Thermo, Thermo Fisher Scientific, Inc.; sc, Santa Cruz Biotechnology, Inc., ON, overnight (ON).