Figure 3.

LEMD1 enhances cell migration and invasion in TC cell lines. (A) Wound healing assays were used to detect cell migration in Si-LEMD1-transfected TC cells (scale bar, 400 µm). (B) Relative quantification of migration rate in wound healing assays. Migration rate=(0 h wound area-24 h wound area)/0 h wound area × 100%. Transwell (C) migration and (D) invasion assays were used to detect cell migration and invasion in TC cells transfected with Si-LEMD1 or LEMD1 OE vector (scale bar, 100 µm; magnification, ×200). The data are shown as the mean ± SD of three independent experiments. Student's unpaired t-test was used for statistical analyses. **P<0.01, ***P<0.001 and ****P<0.0001 vs. Si-NC or control. LEMD1, LEM domain containing 1; TC, thyroid cancer; siRNA, small interfering RNA; OE, overexpression; NC, negative control.