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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Biochemistry. 2017 May 1;56(18):2417–2424. doi: 10.1021/acs.biochem.7b00010

Figure 2.

Figure 2.

RP HPLC chromatograms of a trityl-on oligonucleotide before and after treatment with RecJ and SSBP. With a 10 μM reaction mixture, each peak was assigned a picomole (pm) value based on its relative abundance. Chromatogram B shows crude [40b (Table 1 of the Supporting Information, section II)] product before treatment with 19.5% failure product (II), 17.7% prematurely detritylated FLP (III), and 62.8% trityl-on FLP (IV). Following treatment with 60 units of RecJ and 0.5 μg of SSBP (37 °C for 60 min), chromatogram A shows 32.6% hydrolysis product, 1.9% remaining failure product (II), 2.9% prematurely detritylated FLP (III), and 62.6% trityl-on FLP (IV). Numbers on each chromatogram indicate where sample peaks were detected.