Table 1.
Summary of RecJ Processivity of Trityl-On Crude Samples over Time (30 and 60 min)a
| total digested (pmol) | failures digested (pmol) | percent failure decrease | percent purity increase | |||||
|---|---|---|---|---|---|---|---|---|
| 30 units of RecJ | 30 units of RecJ, 0.5 μg of SSBP | 30 units of RecJ | 30 units of RecJ, 0.5 μg of SSBP | 30 units of RecJ | 30 units of RecJ, 0.5 μg of SSBP | 30 units of RecJ | 30 units of RecJ, 0.5 μg of SSBP | |
| 30 min | ||||||||
| average | 14 | 36 | 11 | 25 | 22 | 49 | 2 | 6 |
| 60 min | ||||||||
| average | 22 | 53 | 17 | 35 | 31 | 53 | 4 | 8 |
a
Each condition tested the ability of RecJ to digest 5′ hydroxylated ssDNA generated from trityl-on samples starting with an average crude purity of 81% (based on 99.5% CE for 10 samples, each with ∼500 pmol of total material). Each time point (30 and 60 min incubation) shows an average processivity for 30 units of RecJ with or without 0.5 μg of SSBP. See also Tables 2 and 3 of the Supporting Information.