Table 7.
Ingredients and detailed chemical composition of the experimental diets
| Diets | ||||
|---|---|---|---|---|
| Control | AP | AP + R | AP + L | |
| Ingredients (g/kg, as fed basis) | ||||
| Wheat | 439 | 460 | 460 | 458 |
| Corn | 150 | 170 | 170 | 170 |
| Soybean meal 48 | 250 | 110 | 110 | 110 |
| Whey powder | 100 | 100 | 100 | 100 |
| Soybean oil | 30 | 30 | 30 | 30 |
| Spirulina | 0 | 100 | 100 | 100 |
| Rovabio® Excel AP | – | – | 0.05 | – |
| Lysozyme | – | – | – | 0.1 |
| L-lysine | 5 | 6 | 6 | 6 |
| DL-methionine | 1 | 1 | 1 | 1 |
| L-threonine | 1 | – | – | – |
| Calcium carbonate | 5 | 6 | 6 | 6 |
| Dicalcium phosphate | 13 | 12 | 12 | 12 |
| Sodium chloride | 3 | 2 | 2 | 2 |
| Vitamin-mineral complex1 | 3 | 3 | 3 | 3 |
| Metabolizable energy (kcal/kg DM)2 | 3.738 | 3.809 | 3.789 | 3.818 |
| Chemical composition (g/100 g, as fed basis) | ||||
| DM | 89.8 | 90.0 | 90.0 | 90.0 |
| CP | 17.9 | 18.1 | 17.9 | 17.8 |
| NDF | 14.6 | 11.9 | 11.9 | 11.8 |
| ADF | 4.21 | 3.97 | 4.06 | 3.94 |
| Ash | 5.11 | 4.76 | 4.89 | 4.75 |
| Crude fat | 5.28 | 5.62 | 5.87 | 5.80 |
| Fatty acid composition (% total fatty acids) | ||||
| 12:0 | 0.123 | 0.122 | 0.146 | 0.140 |
| 14:0 | 0.396 | 0.479 | 0.520 | 0.531 |
| 16:0 | 13.5 | 17.8 | 18.3 | 19.3 |
| 16:1c9 | 0.140 | 0.817 | 0.875 | 0.836 |
| 18:0 | 3.17 | 3.19 | 3.32 | 3.62 |
| 18:1c9 | 24.1 | 21.1 | 20.5 | 20.1 |
| 18:1c11 | 1.30 | 1.23 | 1.28 | 1.27 |
| 18:2n-6 | 48.6 | 43.1 | 42.0 | 39.1 |
| 18:3n-3 | 4.55 | 4.17 | 4.31 | 4.22 |
| 20:0 | 0.364 | 0.325 | 0.324 | 0.323 |
| 20:1c11 | 0.298 | 0.572 | 0.559 | 0.820 |
| 22:0 | 0.365 | 0.365 | 0.365 | 0.421 |
| Pigments (μg/g) | ||||
| Chlorophyll-a3 | 2.70 | 108 | 112 | 132 |
| Chlorophyll-b4 | 4.97 | 14.6 | 13.0 | 17.5 |
| Total chlorophylls5 | 7.67 | 122 | 125 | 149 |
| Total carotenoids6 | 2.41 | 11.6 | 12.6 | 13.0 |
| Total chlorophylls and total carotenoids7 | 10.1 | 134 | 138 | 162 |
| Diterpene profile (μg/g) | ||||
| β-Carotene | 0.160 | 3.57 | 3.14 | 2.15 |
| α-Tocopherol | 7.41 | 12.0 | 12.3 | 12.9 |
| β-Tocopherol | 0.676 | 0.254 | 0.215 | 0.213 |
| γ-Tocopherol | 1.054 | 0.997 | 1.052 | 0.925 |
| α-Tocotrienol | 1.092 | 0.504 | 0.923 | 0.994 |
Dietary treatments: cereal and soybean meal-based diet (control); basal diet with 10% of Arthrospira platensis (AP); basal diet with 10% of Arthrospira platensis supplemented with 0.005% of Rovabio® (AP + R); basal diet with 10% of Arthrospira platensis supplemented with 0.01% of lysozyme (AP + L)
1Premix provided per kg of complete diet: vitamin A, 6500 UI; vitamin D3, 1500 UI; vitamin E, 15 mg; vitamin K3, 1 mg; vitamin B1, 1 mg; vitamin B2, 3 mg; vitamin B6, 2 mg; vitamin B12, 0.02 mg; pantothenic acid, 10 mg; nicotinic acid, 15 mg; folic acid, 0.5 mg, biotin, 0.03 mg; betaine, 115 mg; vitamin C, 20 mg; Copper, 100 mg; iron, 100 mg; iodine, 0.5 mg; manganese 50 mg; selenium, 0.15 mg; zinc, 100 mg; butylated hydroxytoluene, 3 mg
2Metabolizable energy (kcal/kg DM) = 4412–11,06 × Ash (g/kg DM) + 3,37 × Crude Fat (g/kg DM) − 5,18 × ADF (g/kg DM) [63]
3Chlorophyll-a = 11.24 × A662 nm - 2.04 × A645 nm
4Chlorophyll-b = 20.13 × A645 nm - 4.19 × A662 nm
5Total chlorophylls (Ca + b) = 7.05 × A662 nm + 18.09 × A645 nm
6Total carotenoids (Cx + c) = (1000 × A470 nm - 1.90 × Ca - 63.14 × Cb)/214
7Total chlorophylls and carotenoids = (Ca + b) + (Cx + c)