Fig. 9.

Effects of ICA on the migration, invasion, and apoptosis of SiHa cells. a CCK-8 assays were performed to demonstrate cell viability in ICA-treated SiHa cells. b Transwell assays were performed to examine the effects of ICA on SiHa cells migration and invasion. c SiHa cell apoptosis was determined by flow cytometer analysis after ICA treatment. d The expression levels of immunosuppressive factors were determined by ELISA assay. e Western blotting was performed to detect the expression levels of TLR4, MyD88, NF-κB p65, Wnt 1 β-catenin, P16, P53, Ki67, Survivin, Bax, Bcl-2, c-Myc, E-cadherin, and Vimentin in ICA-treated SiHa cells. Data are expressed as the mean ± SD. ^*P < 0.05; ^**P < 0.01 vs control group