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. 2021 Apr 14;21:122. doi: 10.1186/s12906-021-03286-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 10 — a Variable Importance Projection (VIP) score plot detecting the PLSDA identified important biomarkers (b) Significant features identified by random forest, ranked by the mean decrease in the classification accuracy during the study of the effect of Portulaca oleracea seed extract (POS; 200 or 400 mg/kg) on acrylamide (ACR) intoxicated rat model. 17β3-HSD; hydroxysteroid 17-beta dehydrogenase 3, CYP11A1; cytochrome P450, family 11 subfamily a polypeptide 1, GSH; Glutathione, MDA; Malondialdehyde, N_spermatozoa; normal spermatozoa percentage, PCNA; Caspase-3 and proliferating cell nuclear antigen, SOD; superoxide dismutase, wt; weight. The coloured boxes on the right indicate the relative regulation of the corresponding marker in each experimental group