Table 4.
Workflow scheme
| No | Material | c (SeNPs) (µg/ml) | c (FGF2-STAB®) (µg/ml) | Analysis | Summary |
|---|---|---|---|---|---|
| 1 | Collagen/Chitosan scaffold | 0; 2; 10; 20 | 0; 2 |
AB assay CAM assay |
Antagonistic effect of SeNPs and FGF2-STAB® at high concentrations of SeNPs (10 and 20 µg/ml) |
| 2 | Solution | 0; 1; 5; 10; 50; 100 | / |
AB assay IC80 calculation |
IC80 is 5.6 µg/ml of SeNPs for HDF |
| 3 | Solution | / | 0; 0.01; 0.05; 0.1; 0.5; 1 | AB assay | Positive effect on fibroblast metabolic activity at a concentration as low as 0.01 µg/ml |
| 4 | Solution | 1; 10 | 0; 0.01; 0.1; 1 | AB assay | Synergistic effect of SeNPs and FGF2-STAB® was confirmed in solution; the best combination of agents is 0.01–1 µg/ml of SeNPs and 0.01–1 µg/ml of FGF2-STAB® |
| 0; 0.01; 0.1; 1; 10 | 0.1; 1 | ||||
| 5 | Collagen/Chitosan scaffold | 0; 0.5; 1; 5 | 0; 1 |
AB assay, Life/dead assay Broth method Morphology and pore size analysis |
Optimal concentration for Collagen/Chitosan scaffold enrichment is 0.5 µg/ml of SeNPs and 1 µg/ml of FGF2-STAB® |