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. 2021 Apr 13;17:41. doi: 10.1186/s13007-021-00743-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Example of a fresh V. macrocarpon bud in longitudinal section, obtained MRI images at evaluated temperatures, and plots of MGVs by defined ROIs. Fresh longitudinal section of V. macrocarpon bud indicating main anatomical structures (a) (B = bark; BA = bud axis; IB = inner bud (apical meristem and flower primordia); P = pith; S = bud scales; VT = vascular tissue). Longitudinal MR images obtained at 20, − 7, − 14, and − 21 °C (b–e, respectively) from specimens collected in November 2018 (n = 14). Images are close-ups of averages of 23 scans with a field of view of 30 × 30 mm with 512 × 512 pixels. Higher MGV (brighter) areas of images indicate unfrozen tissues. Evaluated ROIs of V. macrocarpon bud (f): blue = outer bud; red = inner bud; green = stem. Plot of changes in MGV for each ROI by temperature exposure (G), originally published in Villouta et al. [19]. Vertical error bars represent the standard error of the mean. Scale bar in A, B, and F equals 1 mm