Fig. 4.

CircPTPRA downregulated MYC and FSCN1 expression via interacting with IGF2BP1 in BC cells. a Heat map showing the differentially expressed genes upon circPTPRA overexpression in T24T and EJ cells. Each sample contained a mixture of three repeats. b Venn diagram indicating the discovery of downregulated mRNAs collection in T24T and EJ cells (filtered by fold change <− 2 and p-value < 0.05). c and d qRT-PCR analysis (c) and western blot analysis (d) comparing circPTPRA-overexpressed BC cells with their respective control cells were shown for relative expression of MYC, FSCN1, and HSPA6. GAPDH was used as a loading control. e and f qRT-PCR analysis and western blot assay showing the expression of MYC, FSCN1, and IGF2BP1 in T24T and EJ cells stably transfected with vector or circPTPRA, and co-transfected with IGF2BP1 or control. Data are presented as the means ± SEM of three independent experiments. **, P < 0.01