Figure 6.
Immunohistochemical comparison of the effects of the intake of HFD (11% fat) versus regular chow diet (5% fat) on the expression of PCSK9 and LDLR proteins in the hormone-dependent breast tumors. IHC visualization of the expression of PCSK9 in BT-474 breast tumor tissues collected from mice fed on regular chow diet (A) and HFD (B). IHC visualization of the expression of LDLR in BT-474 breast tumor tissues collected from regular chow diet (C) and HFD (D) fed mice. (E) Negative control stained BT-474 breast tumor tissue without antibodies. Data show high differentiated and expression levels of PCSK9 and LDLR (brown color) in in breast tumor tissues collected from HFD fed mice versus regular chow diet fed mice. Magnification 10X. Scale bars represent 100 μm.