Table 1.
Effect of HOCl on some AO enzymes
Control | 0.05 mM | 0.1 mM | 0.25 mM | 0.5 mM | 1.0 mM | 2.5 mM | |
---|---|---|---|---|---|---|---|
CAT | 20.43 ± 1.78 | 21.61 ± 2.85 | 23.78 ± 1.71a | 25.67 ± 3.10a | 28.71 ± 2.71a | 30.94 ± 3.81a | 35.29 ± 4.77a |
SOD | 14.36 ± 1.01 | 15.87 ± 1.70 | 17.45 ± 1.60a | 19.21 ± 2.16a | 21.84 ± 2.59a | 23.23 ± 2.48a | 26.09 ± 3.51a |
GPx | 90.61 ± 8.58 | 93.92 ± 7.33 | 100.01 ± 7.51a | 106.1 ± 6.76a | 116.13 ± 8.88a | 161.13 ± 11.09a | 210.58 ± 17.79a |
GST | 14.37 ± 1.11 | 15.13 ± 1.52 | 16.58 ± 1.72a | 17.86 ± 1.91a | 20.16 ± 1.85a | 23.27 ± 1.97a | 26.41 ± 2.34a |
GR | 5.44 ± 0.32 | 3.98 ± 0.41a | 2.95 ± 0.18a | 2.15 ± 0.17a | 1.66 ± 0.14a | 1.10 ± 0.10a | 0.93 ± 0.07a |
TR | 29.09 ± 3.46 | 24.90 ± 1.44a | 22.80 ± 2.21a | 20.29 ± 1.76a | 19.9 ± 1.55a | 16.15 ± 1.25a | 14.50 ± 1.36a |
Specific activity of SOD is in units/mg Hb (one unit is the amount which causes 50% inhibition of pyogallol oxidation in a reaction volume of 3 ml). Specific activities of CAT, GPx, GST, GR and TR are in nmoles/min/mg Hb.
aSignificantly different from control.