Figure 3 .

GAS activated the Nrf2-ARE pathway in the kidneys of mice. (A) Western blot analysis of the NF-κB p65, TNF-α and IL-6 proteins in the kidneys. (B) Relative density analysis of the Nrf2 protein bands. (C) Relative density analysis of the HO-1 protein bands. (D) Relative density analysis of the NQO1 protein bands. β-actin was probed as an internal control in relative density analysis. The vehicle control is set as 1.0. Data are expressed as mean ± S.E.M. and representative of at least three independent experiments (individual animals). All values are expressed as mean ± S.E.M. ^##P < 0.05, compared with the control group; ^**P < 0.05, vs. Pb-treated group.