Figure 4 .

GAS inhibited Pb-induced inflammation in the kidneys of mice. (A) Western blot analysis of the NF-κB p65, TNF-α and IL-6 proteins in the kidneys. (B) Relative density analysis of the NF-κB p65 bands. (C) Relative density analysis of the TNF-α protein bands. (D) Relative density analysis of the IL-6 protein bands. β-actin and Lamin A were probed as the internal control in relative density analysis. The vehicle control is set as 1.0. Data are expressed as mean ± S.E.M. and representative of at least three independent experiments (individual animals). All values are expressed as mean ± S.E.M. ^##P < 0.05, compared with the control group; ^**P < 0.05, vs. Pb-treated group.