Figure 4.

effect of black pepper methanol extract and piperine on the morphology and proliferation of K-562 cells. (a) Representative microscopic images of K-562 cells treated with respective phytochemicals at IC₂₅ and IC₅₀ equivalent concentrations for 48 h followed by trypan blue staining. (b) Bar graph representing the relative percentage count of K-562 cells as treated in (a) by trypan blue staining. Results are the mean ± SEM of three independent experiments. * represents P < 0.05 as compared with the control group. BP-M, black pepper methanol extract; PIP, piperine; IC₂₅ and IC₅₀, IC₂₅ and IC₅₀ equivalent concentrations, respectively.