Figure 7.

effect of black pepper methanol extract and piperine (IC₂₅ and IC₅₀, 48 h) in regulating the transcription of some (anti)apoptotic genes in K-562 cells. Representative semi quantitative PCR images indicating the transcriptional patterns of various caspase, pro- and anti-apoptotic genes in response to (a) black pepper methanol extract and (b) piperine. β-actin was used as a gel loading control. Bar graph below respective gel images represent fold change in expression as compared to the respective vehicle-treated control group. (c) The relative quantification of mRNA expression of caspase-9, caspase-3 and PCNA genes using RT-qPCR in K-562 cells after treatment with black pepper methanol extract and piperine. Results are the mean ± SEM of three independent experiments. * indicates P < 0.05 as compared with the respective control groups for each genes. BP-M, black pepper methanol extract; PIP, piperine; IC₂₅ and IC₅₀, IC₂₅ and IC₅₀ equivalent concentrations, respectively, at 48 h.