Figure 8.

effect of black pepper methanol extract and piperine (IC₂₅ and IC₅₀, 48 h) in regulating the translation of (anti)apoptotic genes in K-562 cells. Representative protein expression patterns of various caspase, pro- and anti-apoptotic genes as determined by immunoblot analysis in response to (a) black pepper methanol extract and (b) piperine. GAPDH was used as a gel loading control for immunoblot analysis. Bar graph below respective gel images represent fold change in expression as compared with the respective vehicle-treated control group. Results are the mean ± SEM of three independent experiments. * indicates P < 0.05 as compared with the respective control group in each protein. BP-M, black pepper methanol extract; PIP, piperine; IC₂₅ and IC₅₀, IC₂₅ and IC₅₀ equivalent concentration, respectively, at 48 h.