Table 1.
details of oligonucleotide primer sequences used in this study for PCR
| Name of gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Product size (bp) | Annealing temp (°C) |
|---|---|---|---|---|
| Caspase-3 (>NM_004346.4) | GGTTCATCCAGTCGCTTTGTG | GCGTCAAAGGAAAAGGACTCA | 121 | 60.0 |
| Caspase-9 (>NM_001229.5) | AGGCCCCATATGATCGAGGA | GGCCTGTGTCCTCTAAGCAG | 139 | 60.3 |
| Bax (>NM_138763.4) | CCTGTGCACCAAGGTGCCGGAACT | CCACCCTGGTCTTGGATCCAGCCC | 99 | 68.2 |
| Bcl-2 (>NM_000657.3) | TTGTGGCCTTCTTTGAGTTCGGTG | GGTGCCGGTTCAGGTACTCAGTCA | 114 | 60.9 |
| PCNA (>NM_002592.2) | GTTACTGAGGGCGAGAAGCG | GGCGGGAAGGAGGAAAGTCT | 167 | 61.3 |
| β-Actin (>NM_001101.5) | TCACCCACACTGTGCCCCATCTACGA | CAGCGGAACCGCTCATTGCCAATGG | 296 | 65.0 |
| β-Actin (>NM_001101.5) (RT-qPCR) | TACGCCAACACAGTGCTGTCT | CTGCATCCTGTCGGCAATG | 63 | 60.0 |