. 2021 Mar 23;10(2):292–298. doi: 10.1093/toxres/tfab010

Table 1.

Effect of 2NS on MDA level and activities of different antioxidant enzymes in kidney and liver tissues of C. punctatus after different hours of exposure

MDA level (n moles MDA/mg protein)
Tissue		24 h	48 h	72 h	96 h	10 days	20 days	30 days
Kidney	Control	1.25 ± 0.03^a,p	1.31 ± 0.09^a,p	1.44 ± 0.18^a,p	1.44 ± 0.18^a,p	1.57 ± 0.18^a,p	1.36 ± 0.22^a,p	1.44 ± 0.18^a,p
	0.16 mg/15 g b.w.	1.75 ± 0.04^b,p	1.89 ± 0.06^b,p	2.39 ± 0.31^a,p	2.61 ± 0.38^a,p	2.56 ± 0.41^a,p	1.58 ± 0.09^a,p	1.52 ± 0.15^a,p
	0.33 mg/15 g b.w.	2.74 ± 0.04^c,pq	3.29 ± 0.16^c,qr	4.36 ± 0.19^b,r	5.99 ± 0.35^b,s	4.14 ± 0.38^b,r	2.71 ± 0.26^b,pq	1.86 ± 0.12^a,p
Liver	Control	2.58 ± 0.29^a,p	2.52 ± 0.07^a,p	2.69 ± 0.04^a,p	2.64 ± 0.03^a,p	2.62 ± 0.05^a,p	2.72 ± 0.52^a,p	2.65 ± 0.02^a,p
	0.16 mg/15 g b.w.	2.19 ± 0.06^a,pq	2.72 ± 0.12^a,pqr	3.24 ± 0.13^a,qr	3.7 ± 0.19^b,r	2.87 ± 0.5^a,pqr	2.29 ± 0.1^a,pq	2.13 ± 0.13^a,p
	0.33 mg/15 g	4.54 ± 0.18^b,pq	3.89 ± 0.04^b,pqr	4.21 ± 0.29^b,pq	4.93 ± 0.05^c,p	3.4 ± 0.2^a,qrs	2.94 ± 0.48^a,rs	2.38 ± 0.25^a,s
Catalase activity/mg protein
Kidney	Control	559.9 ± 11.49^a,p	541.5 ± 8.55^a,p	546.75 ± 4.43^a,pq	573.03 ± 15.85^a,qr	5.81.2 ± 3.26^a,r	574.39 ± 5.8^a,qr	565.19 ± 14.56^a,pq
	0.16 mg/15 g b.w.	513.47 ± 6.49^ab,pq	508.03 ± 4.39^ab,pq	506.28 ± 3.32^a,pq	452.07 ± 21.31^b,q	508.29 ± 17.44^b,p	530.16 ± 23.82^a,pq	535.58 ± 22.97^a,p
	0.33 mg/15 g	467.92 ± 15.53^b,pq	469.27 ± 27.1^b,p	421.44 ± 17.06^b,p	407.73 ± 0.33^b,p	506.28 ± 2.3^b,p	528.16 ± 25.17^a,p	528.77 ± 8.35^a,q
Liver	Control	554.56 ± 27.82^a,p	555.76 ± 28.02^a,p	562.64 ± 30.29^a,pq	555.76 ± 28.02^a,qr	547.76 ± 27.66^a,r	561.72 ± 29.73^a,qr	558.53 ± 28.67^a,pq
	0.16 mg/15 g b.w.	408.81 ± 3.55^ab,pq	380.66 ± 5.8^ab,pq	435 ± 10.08^a,pq	441.5 ± 5.57^b,q	421.81 ± 5.5^b,pq	508.38 ± 23.82^a,pq	528.16 ± 3.42^a,p
	0.33 mg/15 g	329.61 ± 19.49^b,pq	355.46 ± 18.82^b,p	335.47 ± 16.83^b,p	321.9 ± 15.37^b,p	354.71 ± 21.99^b,p	322.05 ± 15.85^a,p	444.72 ± 15.56^a,q
SOD (U/mg protein)
Kidney	Control	18.37 ± 0.69^a,p	18.06 ± 0.96^a,p	17.69 ± 1.3^a,p	18.28 ± 0.76^a,p	18.3 ± 0.75^a,p	18.9 ± 0.41^a,p	18.28 ± 0.76^a,p
	0.16 mg/15 g b.w.	16.88 ± 0.66^ab,pq	15.22 ± 0.61^ab,pqr	13.32 ± 0.5^b,r	13.22 ± 0.56^b,r	14.7 ± 0.56^b,qr	16.63 ± 0.36^b,pq	17.44 ± 0.27^a,p
	0.33 mg/15 g	15.44 ± 0.44^b,pq	13.41 ± 0.65^b,qrs	11.62 ± 0.66^b,s	11.26 ± 0.61^b,s	12.77 ± 0.53^b,rs	14.47 ± 0.38^c,qr	17.36 ± 0.31^a,p
Liver	Control	17.73 ± 0.49^a,p	17.32 ± 0.87^a,p	17.56 ± 0.65^a,p	18.39 ± 0.3^a,p	17.37 ± 0.83^a,p	17.04 ± 1.15^a,p	18.67 ± 0.53^a,p
	0.16 mg/15 g b.w.	17.61 ± 0.32^a,p	14.51 ± 0.58^ab,q	13.34 ± 0.45^b,rs	11.4 ± 0.74^b,s	13.82 ± 0.48^b,qrs	15.62 ± 0.73^a,pqr	16.55 ± 0.64^ab,pq
	0.33 mg/15 g	14.67 ± 0.35^b,p	12.56 ± 0.64^b,qr	10.88 ± 0.05^b,r	10.78 ± 0.05^b,r	11.68 ± 0.48^b,r	13.74 ± 0.51^a,pq	15.59 ± 0.4^b,p
GST activity (n moles of CDNB-GSH conjugate formed/min/mg protein)
Kidney	Control	247.85 ± 6.46^a,p	262.04 ± 2.91^a,p	256.34 ± 5.12^a,p	266.22 ± 7.37^a,pq	255.45 ± 1.89^a,q	265.54 ± 8.58^a,pq	268.44 ± 7.25^a,pq
	0.16 mg/15 g b.w.	290.97 ± 3.45^ab,pqrs	313.45 ± 3.15^b,qrs	340.24 ± 8.53^b,s	362.47 ± 6.2^b,rs	327.03 ± 16.53^a,pq	306.11 ± 2.16^a,pq	284.77 ± 15.98^a,p
	0.33 mg/15 g	296.41 ± 2.52^b,qr	334.11 ± 9.52^b,r	357.9 ± 17.4^b,r	391.97 ± 4.67^b,r	341.33 ± 5.82^a,pq	320.63 ± 6.06^a,q	293.87 ± 6.17^a,q
Liver	Control	377.15 ± 5.61^a,p	383.08 ± 0.85^a,p	382.8 ± 4.02^a,p	363.04 ± 9.03^a,pq	319.13 ± 9.34^a,q	345.61 ± 22.76^a,pq	351.18 ± 6.68^a,pq
	0.16 mg/15 g b.w.	422.71 ± 14.87^ab,pqrs	432.9 ± 14.36^b,qrs	486.11 ± 21.73^b,s	457.16 ± 22.37^b,rs	384.2 ± 4.02^a,pqr	361.33 ± 11^a,pq	353 ± 14.85^a,p
	0.33 mg/15 g	458.99 ± 10^b,qr	461.74 ± 9.98^b,r	507.87 ± 24.85^b,r	486.08 ± 13.96^b,r	384.4 ± 28.49^a,pq	380.4 ± 6.92^a,q	370.4 ± 6.92^a,q

The degree of significance was p ≤ 0.05.

Results are provided in mean values along with standard errors (SE). Different letters a, b, c signify effect of concentration and p, q, r and s signify the effect of duration.