Table 5.
Band intensities for selected bands and intensities of specific peaks presenting protein structure of control and 2NS treated tissues of C. punctatus
| The average FTIR absorption intensity ratios for selected bands | |||||||
|---|---|---|---|---|---|---|---|
| Kidney | Liver | ||||||
| Ratio of bands | Control | Group 1 | Group 2 | Control | Group 1 | Group 2 | |
| I1543/I3284 | 1.55 ± 0.028a | 1.74 ± 0.023b | 1.52 ± 0.012a | 1.61 ± 0.008a | 1.61 ± 0.005a | 1.44 ± 0.008b | |
| I2956/2853 | 1.34 ± 0.023a | 1.14 ± 0.012b | 1.06 ± 0.005c | 1.05 ± 0.012a | 1.03 ± 0.020a | 1.01 ± 0.016a | |
| I1543/I1652 | 1.01 ± 0.005a | 0.97 ± 0.050b | 1.09 ± 0.050c | 1.01 ± 0.006a | 0.90 ± 0.005b | 0.86 ± 0.017b | |
| I1082/I1543 | 0.75 ± 0.008a | 1.13 ± 0.008b | 1.14 ± 0.008b | 0.75 ± 0.029a | 1.04 ± 0.007b | 1.17 ± 0.005c | |
| The peak centers and the absorption intensity of main protein secondary structures | |||||||
| Kidney | Liver | ||||||
| Absorption intensity | |||||||
| Structure | Peak centers (cm−1) | Control | Group 1 | Group 2 | Control | Group 1 | Group 2 |
| α-Helix | 1654 | 0.38 ± 0.005a | 0.34 ± 0.008b | 0.31 ± 0.005c | 0.33 ± 0.008a | 0.34 ± 0.005a | 0.32 ± 0.005a |
| β-Sheet | 1625, 1632, 1684 | 0.98 ± 0.016a | 0.86 ± 0.018b | 0.84 ± 0.019b | 0.92 ± 0.011a | 0.77 ± 0.018b | 0.77 ± 0.018b |
| Turns and bends | 1662, 1669 | 0.54 ± 0.008a | 0.55 ± 0.016a | 0.47 ± 0.010b | 0.52 ± 0.010a | 0.47 ± 0.013b | 0.46 ± 0.008b |
| Random coils | 1647 | 0.41 ± 0.008a | 0.38 ± 0.005b | 0.35 ± 0.005b | 0.40 ± 0.005a | 0.34 ± 0.012b | 0.34 ± 0.008b |
The degree of significance was P ≤ 0.05.
Results are provided in mean values along with standard errors (SE). Different letters a, b, c signify effect of treatment at the same time interval (one-way ANOVA).