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. 2021 Mar 23;10(2):292–298. doi: 10.1093/toxres/tfab010

Table 5.

Band intensities for selected bands and intensities of specific peaks presenting protein structure of control and 2NS treated tissues of C. punctatus

The average FTIR absorption intensity ratios for selected bands
Kidney Liver
Ratio of bands Control Group 1 Group 2 Control Group 1 Group 2
I1543/I3284 1.55 ± 0.028a 1.74 ± 0.023b 1.52 ± 0.012a 1.61 ± 0.008a 1.61 ± 0.005a 1.44 ± 0.008b
I2956/2853 1.34 ± 0.023a 1.14 ± 0.012b 1.06 ± 0.005c 1.05 ± 0.012a 1.03 ± 0.020a 1.01 ± 0.016a
I1543/I1652 1.01 ± 0.005a 0.97 ± 0.050b 1.09 ± 0.050c 1.01 ± 0.006a 0.90 ± 0.005b 0.86 ± 0.017b
I1082/I1543 0.75 ± 0.008a 1.13 ± 0.008b 1.14 ± 0.008b 0.75 ± 0.029a 1.04 ± 0.007b 1.17 ± 0.005c
The peak centers and the absorption intensity of main protein secondary structures
Kidney Liver
Absorption intensity
Structure Peak centers (cm−1) Control Group 1 Group 2 Control Group 1 Group 2
α-Helix 1654 0.38 ± 0.005a 0.34 ± 0.008b 0.31 ± 0.005c 0.33 ± 0.008a 0.34 ± 0.005a 0.32 ± 0.005a
β-Sheet 1625, 1632, 1684 0.98 ± 0.016a 0.86 ± 0.018b 0.84 ± 0.019b 0.92 ± 0.011a 0.77 ± 0.018b 0.77 ± 0.018b
Turns and bends 1662, 1669 0.54 ± 0.008a 0.55 ± 0.016a 0.47 ± 0.010b 0.52 ± 0.010a 0.47 ± 0.013b 0.46 ± 0.008b
Random coils 1647 0.41 ± 0.008a 0.38 ± 0.005b 0.35 ± 0.005b 0.40 ± 0.005a 0.34 ± 0.012b 0.34 ± 0.008b

The degree of significance was P ≤ 0.05.

Results are provided in mean values along with standard errors (SE). Different letters a, b, c signify effect of treatment at the same time interval (one-way ANOVA).