Table 3.
comparison of different concentrations of PEBHAE in MN assay
| Cyto B (4 μg/ml) for 28 h | |||||||
|---|---|---|---|---|---|---|---|
| Mono N | Bi N | Multi N | MN | CBPI | RI | Cytostasis (%) | |
| NC | 400 | 160 | 8 | 12 | 1/31 | ||
| Positive control (cisplatin, 10 μM) | 612 | 72 | 7 | 135 | 1/12 | 46/93 | 60/99 |
| PEBHAE only (200 μg/ml) | 436 | 116 | 5 | 29 | 1/23 | 76/74 | 27/48 (***) |
| Cisplatin 10 μM + | |||||||
| PEBHAE, 10 μg/ml | 460 | 96 | 12 | 97 | 1/22 | 63/39 | 31/97 (**) |
| PEBHAE, 20 μg/ml | 474 | 96 | 8 | 93 | 1/20 | 63/16 | 37/87 (*) |
| PEBHAE, 50 μg/ml | 481 | 86 | 9 | 88 | 1/18 | 56/80 | 42/50 |
| PEBHAE, 75 μg/ml | 492 | 98 | 8 | 82 | 1/20 | 64/70 | 37/12 (*) |
| PEBHAE, 100 μg/ml | 486 | 99 | 10 | 65 | 1/20 | 65/13 | 36/81 (**) |
| PEBHAE, 200 μg/ml | 489 | 93 | 8 | 53 | 1/19 | 61/62 | 40/62 (*) |
Data are expressed as mean (n = 3). Signs (*), (**), and (***) represent statistically significant difference (P < 0.05, P < 0.01, and P < 0.001, respectively) compared with positive control (i.e. cells that received only cisplatin with a concentration of 10 μM). Cyto B, cytochalasin B; Mono N, mono nucleated cell; Bi N, binucleated cell; Multi N, multi-nucleated cell; CBPI, cytokinesis-block proliferation index; RI, replicative index.