Table 1.

Potential identities of samples based on the top three rankings sorted by the “Query Cover” and “Ident” functions in GenBank

	Top three rankings of potential identities in GenBank
	Sorted with descending order of “Query Cover”				Sorted with descending order of “Ident”
Sample†	Query cover (%)	Identity to sample (%)	Microorganism*	Accession number	Query Cover (%)	Identity to sample (%)	Microorganism*	Accession number
WFMax1B (3,902 bp)	67	91	S. zamani	KU244528.1	57	93	S. singaporensis	KU341123.1
	67	89	S. zuoi	KU341120.1	32	92	S. sp. ex Morelia viridis	KC201640.1
	57	93	S. singaporensis	KU341123.1	62	90	S. zuoi	KU341121.1
J1.11 (3,979 bp)	88	96	S. singaporensis	KU341123.1	32	99	S. singaporensis	KY513624.1
	74	96	S. zamani	KU244528.1	74	96	S. zamani	KU244528.1
	73	95	S. zuoi	KU341120.1	63	96	S. zuoi	KU341121.1
C2.5 (4,003 bp)	84	87	B. besnoiti	DQ227420.1	33	99	G. szekelyi	GU479656.1
	66	94	T. gondii	X75453.1	32	98	G. koertingi	GU479647.1
	40	94	H. hammondi	AH008381.2	31	98	G. pannonica	GU479642.1
C2.1 (4,164 bp)	81	88	B. besnoiti	DQ227420.1	31	98	G. janae	GU479644.1
	75	94	T. gondii	X75453.1	30	98	G. pannonica	GU479642.1
	36	94	I. belli	DQ060661.2	30	98	G. koertingi	GU479647.1
C1.9 (4,053 bp)	80	88	B. besnoiti	DQ227420.1	28	98	G. pannonica	GU479642.1
	73	94	T. gondii	X75453.1	28	97	G. janae	GU479644.1
	34	94	I. belli	DQ060661.2	28	97	G. koertingi	GU479647.1

* Genus names: B = Besnoitia; G = Goussia; H = Hammondia; I = Isospora; S = Sarcocystis; T = Toxoplasma.

^†Sequence of WFMax1B was produced from nested PCR of purified primary PCR; J1.11 from Lucigen cloning (nested PCR); C2.5, C2.1, and C1.9 from NEB cloning (Re-PCR).