TABLE OF MATERIALS:
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 1 L Centrifuge bottles, PC/PPCO, super speed, with sealing cap, Nalgene | VWR | 525–0466 | |
| 1 L Pyrex Media Storage Bottle with High Temperature Cap | VWR | 10416–312 | |
| 2,000 mL Erlenmeyer Flask, Narrow Mouth | VWR | 10545–844 | |
| 4–20% mini PROTEAN Precast Protein Gels, 12 well | BIORAD | 4561095 | |
| 4x Laemmli Sample Buffer 10 mL | BIORAD | 1610747 | |
| 50 mL sterile polypropylene centrifuge tubes | VWR | 89049–174 | |
| 7 mL Dounce Tissue Homogenizer with Two Glass Pestles | VWR | 71000–518 | |
| 70 mL polycarbonate bottle assembly | Beckman Coulter Life Sciences | 355622 | |
| Agar Powder | VWR | A10752 | |
| B10P Benchtop pH Meter with pH Probe | VWR | 89231–664 | |
| Barnstead GenPure xCAD Plus UV/UF - TOC (bench version) | ThermoFisher Scientific | 50136146 | |
| Benzonase Nuclease | MilliporeSigma | 70746–3 | |
| EDTA disodium salt dehydrate 99.0–101.0%, crystals, ultrapure Bioreagent Molecular biology grade, J.T. Baker | VWR | 4040–01 | |
| EmulsiFlex-C3 | Avestin, Inc. | ||
| Fiberlite F13–14 × 50cy Fixed Angle Rotor | ThermoFisher Scientific | 75006526 | |
| Hydrochloric acid 6.0 N | VWR | BDH7204 | |
| IBI Scientific Orbital Platform Shaker | Fischer Scientific | 15-453-211 | |
| LB Broth Miller | VWR | 214906 | |
| Lysozyme, Egg White, Ultra Pure Grade | VWR | VWRV0063 | |
| Magnesium chloride hexahydrate | Sigma Aldrich | M2670 | |
| NADH | Sigma Aldrich | 10107735001 | |
| Optima XPN-80 - IVD | Beckman Coulter Life Sciences | A99839 | |
| Pharmco Products PURE ALCOHOL 200 PROOF GL 4/CS | Fischer Scientific | NC1624582 | |
| Phenol Solution, Equilibrated with 10 mM Tris HCI, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology | Sigma - Millipore | P4557 | |
| Pierce Coomassie Plus (Bradford) Assay Kit | Thermofisher | 23238 | |
| Pro-Q emerald 300 Lipopolysaccharide Gel Stain Kit | Thermofisher | P20495 | |
| Proteacease −50, EDTA free | G Biosciences | 786–334 | |
| Proteinase K, Molecular Biology Grade | New England Biolabs | P8107S | |
| Sodium hydroxide >99.99% | VWR | AA45780–22 | |
| Sorval RC 6 Plus Centrifuge | ThermoFisher Scientific | 36-101-0816 | |
| Sucrose | MilliporeSigma | SX1075–3 | |
| SW 41 Ti Swinging-Bucket Rotor | Beckman Coulter Life Sciences | 331362 | |
| Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% (dried basis), ultrapure Bioreagent Molecular biology grade, J.T. Baker | VWR | JT4109–6 | |
| Type 45 Ti Fixed-Angle Titanium Rotor | Beckman Coulter Life Sciences | 339160 | |
| Ultra-Clear Tube, 14 × 89mm | Beckman Coulter Life Sciences | 344059 | |
| Vortex-Genie 2 | VWR | 102091–234 |