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. 2021 Jan 11;217(2):iyaa036. doi: 10.1093/genetics/iyaa036

Figure 2.

Figure 2

Sequence and expression of MYB5a/NEGAN. (A) Alignment of MYB5a, MYB5b, and MYB4. Amino acid sequences were inferred by translating DNA sequences, obtained from the M. l. luteus genome and transcriptome (Edger et al. 2017; Stanton et al. 2017) and from PCR and Sanger sequencing of M. l. variegatus. Black rectangles identify putative Subgroup 6 motifs. Note that this motif, which strongly predicts ability to activate anthocyanin production, is missing from M. l. variegatus exon 3. Gray boxes denote exon boundaries for MYB5a and MYB5b. Created in Geneious 9.1 by Biomatters (www.geneious.com). (B) qRT-PCR estimates of MYB5a/NEGAN and MYB5b expression in M. l. variegatus and M. l. luteus. Each point indicates the mean of three technical replicates, ±95% CI, relative to the reference gene Actin and a reference pool of cDNA.