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. 2021 Jan 11;217(2):iyaa036. doi: 10.1093/genetics/iyaa036

Figure 5.

Figure 5

RNAi knockdown of MYB5a (transcript Mlu 12200) coordinately down-regulates Late Biosynthetic Genes and the entire anthocyanin regulatory network. (A) Transcript Mlu 12200 corresponds to Myb5a/NEGAN. Bars show average read-count (n = 3) of wild-type and RNAi lines respectively, for candidate transcripts Mlu 12200, Mlu 12207, and Mlu 42095. Error bars mark the highest and lowest read counts; only Mlu 12200 has any mapped reads. (B) Differentially expressed anthocyanin biosynthesis genes. Green, EBGs (Early Biosynthetic Genes). Purple, LBGs (Late Biosynthetic Genes). Log-2-fold change (RNAi vs wild-type) is shown on the x axis, and −log 10 of adjusted P-value on the y axis. (C) Transcript level fold change of anthocyanin regulators. Normalized expression fold change is shown in RPKM (per million mapped reads). Error bars represent maximum and minimum expression levels among the samples (n = 3). (D) The six regulatory genes of interest (Myb5a, MlutbHLH1, MlutbHLH2, MlutRTO1, MlutRTO2, and MlutWD40a) are highlighted in the gene expression volcano plot.