Figure 7.

Solid black arrows indicate the direction of gene expression change, following RNAi knockdown of MYB5a/NEGAN in M. l. variegatus. The number of black arrows corresponds to the number of gene copies identified in the transcriptome. Red X, gene expression near zero in the RNAi line; thin black arrow, approximately 3-fold downregulation in the RNAi line; medium black arrow, approximately 9-fold downregulation; thick black arrow, more than 18-fold downregulation. Grey symbols denote positive and negative regulatory interactions. RTO is an R3 MYB protein that inhibits anthocyanin biosynthesis by sequestering bHLH proteins away from the MBW complex.