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. 2021 Feb 3;217(2):iyaa035. doi: 10.1093/genetics/iyaa035

Figure 1.

Figure 1

RTT105 inactivation results in cell lethality in different genetic contexts affecting replisome progression. (A) Members of the PIF1 DNA helicase family are not required for viability of yeast rtt105Δ cells. Tetrad dissection of the diploid strains pif1Δ/PIF1 rtt105Δ/RTT105 and rrm3Δ/RRM3 rtt105Δ/RTT105. In this and subsequent figures, the spores from a given tetrad are in vertical line in a YPD plate. Four representative tetrads are shown after 3 days at 30°C. (B) Genetic interaction of mcm2-1 and cdc17-1 with RTT105. The diploid strains mcm2-1/MCM2 rtt105Δ/RTT105 (left) and cdc17-1/CDC17 rtt105Δ/RTT105 (right) were sporulated and dissected. (C) RTT105 is required for viability in the absence of the replicative function of MRC1 or the Tof1-Csm3 complex. Left, tetrads from diploids heterozygous for mrc1Δ, and for rtt105Δ were dissected and analyzed as in (A). Center, tetrads from mrc1-C14, and rtt105Δ heterozygous diploids were dissected and analyzed. Right, tetrads from diploids heterozygous for tof1Δ, and for rtt105Δ were dissected and analyzed.