Figure 2.

RTT105 shows genetic interactions with components involved in DNA repair by homologous recombination. (A, B) sae2Δ/SAE2 rtt105Δ/RTT105 and mre11Δ/MRE11 rtt105Δ/RTT105 diploid strains were dissected and the resulting spores were incubated at 30°C for 3 days. (C) Left, Rfa1 foci are detected in rtt105Δ cells. Wild-type and rtt105Δ cells encoding Rfa1-CFP were analyzed with differential interference contrast (DIC) (left) and with florescence microscopy (right). Right, numbers indicate the percentage of cells that contained Rfa1-CFP foci. Rfa1 foci were analyzed in asynchronously growing cells. At least 200 cells were analyzed for each strain. (D) Genetic interaction of LIF1, with RTT105. lif1Δ/LIF1 rtt105Δ/RTT105 were dissected. Tetrads were grown at 30°C for 3 days.