Table 2.
Genetic dependence on rtt105Δ155-208 viability. rtt105Δ/RTT105 strains heterozygous for the indicated deletions expressing rtt105Δ155-208 or RTT105 from a centromeric plasmid were sporulated, dissected, and the genotype of the variable spores were determined
| Mutant | Growth defect | Growth defect | Growth defect |
|---|---|---|---|
| with rtt105Δ + | with rtt105Δ + | with rtt105Δ + | |
| rtt105Δ155-208 | RTT105 | Empty vector | |
| orc5-1 | Synthetic lethal | − | Synthetic lethal |
| mcm2-1 | +++ | − | +++ |
| cdc17-1 | Synthetic lethal | − | Synthetic lethal |
| mrc1Δ | Synthetic lethal | − | Synthetic lethal |
| tof1Δ | Synthetic lethal | − | Synthetic lethal |
| ctf18Δ | Synthetic lethal | − | Synthetic lethal |
| rad9Δ | +++ | − | Synthetic lethal |
| rad53-K227A | +++ | − | Synthetic lethal |
| rad52Δ | Synthetic lethal | − | Synthetic lethal |
| mre11Δ | Synthetic lethal | − | Synthetic lethal |
| sae2Δ | +++ | − | +++ |
| lif1Δ | − | − | − |
| scc1-73* | Synthetic lethal | − | Synthetic lethal |
| asf1Δ | +++ | − | +++ |
| sir4Δ | − | − | − |
A “−” represents no effect on growth over the individual single mutants. A “+” represents a synthetic effect on growth. More “+” indicate a more dramatic synthetic effect in comparison with other strain tested. All the analyses were conducted at 30°C excepted for those marked by a “*”, which were conducted at 32°C.