Figure 3.

Comparison of Dollo’s law versus reacquisition of the GAL genes from the CUG-Ser1 clade. (A) Evolutionary trait reconstruction based on a parsimony framework either assuming that traits cannot be regained (left) or that traits can be regained (right). (B) Similarity scores of the Gal1, Gal7, and Gal10 proteins as calculated by protein sequence similarity and the comparisons shown on the left; means with standard deviations are depicted on the right. Raw percent identity values and all other comparisons are shown in Supplementary Figure S6. Comparisons used to calculate similarity scores: A, between species within the clade with potentially transferred GAL genes (recipient clade); B, between the recipient clade and their closest relative with GAL genes; C, between the recipient clade and the potential donor lineage (CUG-Ser1 clade); and D, between the recipient clade and an outgroup lineage. Student’s t-tests of the mean differences in similarity scores between groups are shown (n = 7).