Table 2.

Summary of crispant phenotypes

Targeted gene	Individual	Phenotype
Antp	Figure 2B	Loss of Cu1 eyespot on the ventral side of forewing
	Figure 2E	Complete or partial loss of white eyespot center on the ventral side of hindwings
	Figure 2F	- Loss of Cu1 eyespot on the ventral side of forewing - Transformation of silver scales into brown scales on the dorsal side of forewing
	Supplementary Figure S2A	- Loss of white (and gold) scales of eyespots on the ventral side of hindwing
	Supplementary Figure S2B	- Partial loss of white eyespot center (Cu1 eyespot) on the ventral side of forewing - Transformation of sliver scales into brown scales on the ventral side of forewing - Loss of white and gold scales of eyespot on the ventral side of hindwing
	Supplementary Figure S2C	- Partial loss of Cu1 eyespots on the ventral side of forewings - Loss of white eyespot center on the dorsal side of hindwing
	Supplementary Figure S2D and Figure 2K lower	- Loss of M1 eyespot on the dorsal side of the forewing - Loss of white eyespot center on the dorsal side of the hindwing - Loss of white eyespot centers on the ventral side of hindwing
	Supplementary Figure S2E and Figure 2K upper	- Partial loss of Cu1 eyespot on the ventral side of forewing - Loss of white eyespot centers on the ventral side of the hindwing - Loss of Cu1 eyespot on the dorsal side of the forewing
Ubx	Figure 3A	- Enlargement of Cu1 eyespot on the ventral side of the hindwing - Disappearance of M3 eyespot on the ventral side of the hindwing - Ectopic silver scales were generated on the ventral side of the hindwing
	Figure 3H	- Loss of Rs eyespot on the ventral side of hindwing - Enlargement of M1 eyespot on the ventral side of hindwing - Ectopic eyespots were generated in M1, M2, and Cu1 wing sectors on the dorsal side of the hindwing resembling the ventral side of the forewing
	Supplementary Figure S3A	- Enlargement of M1 eyespot on the ventral side of hindwing *This individual has abnormal gold color spreading from Cu1 eyespot on the ventral side of forewing probably due to damage during wing development
	Supplementary Figure S3B	- Ectopic eyespot was generated in M1 wing sector on the ventral side of hindwing resembling the larger size of the M1 eyespot on the ventral forewing
	Supplementary Figure S3C	- Decrease in the M3 eyespot size on the ventral side of hindwing - Enlargement of Cu1 eyespot on the ventral side of hindwing
	Supplementary Figure S3D	- Ectopic eyespots were generated in a double M1 wing sector on the ventral and dorsal hindwing *This individual is a spontaneous mutant having an extra vein below M1 wing sector, resulting in additional eyespots visible on both sides of the wing. The M1 eyespot on the dorsal surface resembles in size more the ventral forewing M1 eyespot than the dorsal forewing M1 eyespot