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. 2021 Jan 20;217(3):iyab006. doi: 10.1093/genetics/iyab006

Figure 6.

Figure 6

A new suite of TIR1 expression strains for tissue-specific depletion of AID-tagged proteins in C. elegans. (A) Table describing new suite of TIR1::F2A::BFP::AID*::NLS strains. Strain names, promoter driving TIR1, tissue of expression, genotype, and insertion site are provided for each strain. The insertion sites are the genomic loci where the MosI transposon landed in the ttTi4348 and ttTi5605 insertion alleles. We note that our knock-ins were generated using CRISPR/Cas9-mediated genome editing in wild-type animals or in strains stably expressing Cas9 in the germline; there is no MosI transposon in these loci in these genetic backgrounds. Created with BioRender.com. (B) BFP is detected in the expected nuclei of strains expressing TIR1 cassettes driven by col-10p (hypodermis), unc-54p (muscle), ges-1p (intestine), and rgef-1p (neurons). Representative BFP-expressing nuclei are indicated by solid arrows. Scale bars represent 20 µm. Note that the fluorescence signal at the bottom of the muscle image and surrounding the nuclei in the intestinal image is intestinal autofluorescence, indicated by an unfilled arrow with a dashed outline. (C) Functional test of TIR1 activity in a col-10p::TIR1::F2A::BFP::AID*::NLS strain (DV3799). Hypodermal BFP expression is lost when animals are exposed to 1 mM auxin for three hours, but not when similarly grown on control plates.