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. 2021 Jan 20;217(3):iyab006. doi: 10.1093/genetics/iyab006

Figure 7.

Figure 7

NHR-25::GFP::AID*::3xFLAG can be depleted in a cell-specific manner in a strain with undetectable TIR1 expression via a BFP reporter. (A) An anchor cell (AC)-specific TIR1 transgene (cdh-3p::TIR1::F2A::BFP::AID*::NLS) did not produce observable BFP in the AC. Crossing this strain to an nhr-25::GFP::AID*::3xFLAG allele resulted in depletion of NHR-25 in the AC when exposed to 4 mM auxin for 1 hr (indicated by white arrow with black outline). As expected, depletion of NHR-25 was not observed in the neighboring uterine cells or the underlying vulval precursor cells (VPCs). Scale bar represents 5 µm. (B) Quantification of NHR-25::GFP::AID*::3xFLAG in ACs following auxin (K-NAA) treatment. Individual data points from a single replicate with more than 10 animals per condition are presented. The horizontal black bar depicts the mean for each condition; **** indicates P < 0.0001 by a two-tailed unpaired Student’s t-test. P < 0.05 was considered statistically significant. Scale bars represent 5 µm.