Table 1.

Plasmids to support generation of new TIR1 alleles and other transgenes at standardized genetic loci through CRISPR/Cas-based genome editing

Plasmid name	Description	Purpose
pJW1838	SapTrap sgRNA (F + E) vector, K09B11.2 U6 promoter and 3'UTR	Creating new sgRNA (F + E) plasmids)
pJW1839	SapTrap sgRNA (F + E) vector, R07E5.16 U6 promoter	Creating new sgRNA (F + E) plasmids)
pJW1836	promoterless SV40 NLS::mScarlet-I (dpi)::PEST::tbb-2 3'UTR vector	Promoter reporter, test new tissue-specific promoters
pJW1841	promoterless SV40 NLS::mScarlet-I (dpi)::tbb-2 3'UTR vector	Promoter reporter, test new tissue-specific promoters
pJW1849	ttTi4348 site targeting sgRNA (F + E) with K09B11.2 U6 promoter and 3'UTR	CRISPR/Cas9 editing of ttTi4348 insertion site
pJW1850	ttTi5605 site targeting sgRNA (F + E) with K09B11.2 U6 promoter and 3'UTR	CRISPR/Cas9 editing of ttTi5605 insertion site
pJW1851	cxTi10882 site targeting sgRNA (F + E) with K09B11.2 U6 promoter and 3'UTR	CRISPR/Cas9 editing of cxTi10882 insertion site
pJW1882	ttTi4348 site targeting sgRNA (F + E) with R07E5.16 U6 promoter and 3'UTR	CRISPR/Cas9 editing of ttTi4348 insertion site
pJW1883	ttTi5605 site targeting sgRNA (F + E) with R07E5.16 U6 promoter and 3'UTR	CRISPR/Cas9 editing of ttTi5605 insertion site
pJW1884	cxTi10882 site targeting sgRNA (F + E) with R07E5.16 U6 promoter and 3'UTR	CRISPR/Cas9 editing of cxTi10882 insertion site
pJW1947	pes-10Δ minimal promoter::SV40 NLS::mScarlet-I (dpi)::tbb-2 3'UTR vector	Promoter reporter, test new tissue-specific enhancers
pJW1948	pes-10Δ minimal promoter::SV40 NLS::mScarlet-I (dpi)::PEST::tbb-2 3'UTR vector	Promoter reporter, test new tissue-specific enhancers
pTD77	eft-3p::Cas9, R07E5.16 U6::sgRNA targeting LGI site where ttTi4348 Mos is inserted	CRISPR/Cas9 editing of ttTi4348 insertion site
pTD78	eft-3p::Cas9, R07E5.16 U6::sgRNA targeting LGII site where ttTi5605 Mos is inserted	CRISPR/Cas9 editing of ttTi5605 insertion site