Table 2.
eft-3p::TIR1::F2A::BFP::AID*::NLS produces comparable depletion phenotypes to eft-3p::TIR1::mRuby2 for depleting nuclear proteins
| eft-3p::TIR1 transgene | AID-tagged allele | Treatment | % WT developmental rate | % Larval arrest | % Gonadal abnormalities among adults |
|---|---|---|---|---|---|
| TIR1::mRuby2 | n/a | Control | 100 | 0 | 0 |
| TIR1::mRuby2 | n/a | IAA | 100 | 0 | 0 |
| TIR1::mRuby2 | n/a | K-NAA | 100 | 0 | 0 |
| TIR1::2A::BFP | n/a | Control | 100 | 0 | 0 |
| TIR1::2A::BFP | n/a | IAA | 100 | 0 | 0 |
| TIR1::2A::BFP | n/a | K-NAA | 100 | 0 | 0 |
| TIR1::mRuby2 | nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) | Control | 100 | 0 | 0 |
| TIR1::mRuby2 | nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) | IAA | 0 | 100 | 0 |
| TIR1::mRuby2 | nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) | K-NAA | 0 | 100 | 0 |
| TIR1::2A::BFP | nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) | Control | 100 | 0 | 0 |
| TIR1::2A::BFP | nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) | IAA | 0 | 100 | 0 |
| TIR1::2A::BFP | nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) | K-NAA | 0 | 100 | 0 |
| TIR1::mRuby2 | nhr-25(wrd18[nhr-25::GFP^AID*:3xFLAG]) | Control | 100 | 0 | 0 |
| TIR1::mRuby2 | nhr-25(wrd18[nhr-25::GFP^AID*:3xFLAG]) | IAA | 39 | 61 | 100 |
| TIR1::mRuby2 | nhr-25(wrd18[nhr-25::GFP^AID*:3xFLAG]) | K-NAA | 100 | 0 | 100 |
| TIR1::2A::BFP | nhr-25(wrd52[nhr-25::GFP^AID*:3xFLAG]) | Control | 100 | 0 | 0 |
| TIR1::2A::BFP | nhr-25(wrd52[nhr-25::GFP^AID*:3xFLAG]) | IAA | 15 | 85 | 93 |
| TIR1::2A::BFP | nhr-25(wrd52[nhr-25::GFP^AID*:3xFLAG]) | K-NAA | 100 | 0 | 100 |
Synchronized animals (n > 100) of the indicated genotype were grown on the indicated plates (control, IAA, K-NAA). These animals were scored for developmental rate (% animals that were L4 or adults following 48 h at 25°C), larval arrest prior to L4 (animals that failed to reach adulthood after 4 days), and gonadal defects. Data from TIR1::2A::BFP and TIR1::2A::BFP; nhr-23(kry61[nhr-23::AID*::TEV-3xFLAG]) represent two pooled experimental replicates. All other data are from a single experiment.