Table 3.
eft-3p::TIR1::F2A::BFP::AID*::NLS produces comparable depletion phenotypes to eft-3p::TIR1::mRuby2 for depleting cytoplasmic proteins
| eft-3p::TIR1 transgene | AID-tagged allele | Treatment | % WT developmental rate | % L2 arrest | % L3 arrest |
|---|---|---|---|---|---|
| TIR1::mRuby2 | daf-15(re257[daf-15::mNG::AID*]) | Control | 100 | 0 | 0 |
| TIR1::mRuby2 | daf-15(re257[daf-15::mNG::AID*]) | IAA | 0 | 100 | 0 |
| TIR1::mRuby2 | daf-15(re257[daf-15::mNG::AID*]) | K-NAA | 0 | 0 | 100 |
| TIR1::2A::BFP | daf-15(re257[daf-15::mNG::AID*]) | Control | 100 | 0 | 0 |
| TIR1::2A::BFP | daf-15(re257[daf-15::mNG::AID*]) | IAA | 0 | 100 | 0 |
| TIR1::2A::BFP | daf-15(re257[daf-15::mNG::AID*]) | K-NAA | 0 | 0 | 100 |
Synchronized animals (n = 120–240) of the indicated genotype were grown on the indicated plates (control, IAA, K-NAA). Animals were grown at 20°C for 4 days and scored for larval arrest. Wild-type (WT) developmental rate was determined by scoring animals that reached adulthood after 3 days at 20°C. L2 and L3 arrest were determined by animal size and gonadal development.