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. 2021 Mar 25;11:651921. doi: 10.3389/fonc.2021.651921

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Copyright © 2021 Liu, Hou, Hu, Zhou, Chen, Yang and Song

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Function of SLC39A8 and its expression in ccRCC samples and RCC cell lines. (A) Immunohistochemistry for SLC39A8 expression in ccRCC tissues and the corresponding normal tissues. The inserted images are the higher magnification picture of a certain part of the same tissue, ×100 and ×400. (B, C) Reverse transcription-quantitative PCR assays and western blotting experiments of SLC39A8 expression in normal human renal epithelial cell line HK2 and RCC cell lines (786-O, OSRC-2, ACHN, A498 and CAKI-1) and ccRCC patients. (D) Functional enrichment analysis of ZIP family genes by Metascape. The results showed that ZIP family functions in the cellular import of zinc. (E) Cell proliferation assay of 786-O and OSRC-2 cells treated with different concentrations of zinc chloride (0–200 μM) for 48–72 h. (F) The colony formation assay of 786-O and OSRC-2 cells treated with 0–20 μM zinc chloride. The values of each group were presented as the mean ± standard deviation. *P <0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.