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. 2021 Mar 25;11:651921. doi: 10.3389/fonc.2021.651921

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Copyright © 2021 Liu, Hou, Hu, Zhou, Chen, Yang and Song

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SLC39A8/zinc inhibits the proliferation of ccRCC cells in vitro. (A) Quantitative real-time PCR and western blotting experiments of SLC39A8 knockdown in 786-O and OSRC-2 cells. (B, C) Cell proliferation assay and colony formation assay detected the effects of SLC39A8 knockdown with or without zinc supplementation on the proliferation of 786-O and OSRC-2 cells. (D) Quantitative real-time PCR and western blotting experiments of SLC39A8 overexpression in 786-O and OSRC-2 cells. (E, F) Cell proliferation assay and colony formation assay detected the effects of SLC39A8 overexpression with or without zinc supplementation on the proliferation of 786-O and OSRC-2 cells. The values of each group were presented as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.