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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Stem Cell Res. 2019 Feb 15;36:101408. doi: 10.1016/j.scr.2019.101408

Fig. 1. Characterization of TRNDi005-A iPSC line.

Fig. 1.

A) Detection of compound heterozygous mutations of p. R61W in exon 2 and p. WT405del in exon 11 of the GALNS gene. B) Cytogenetic analysis showing a normal karyotype (46, XY). C) Left: Phase contrast imaging of TRNDi005-A colonies grown on Matrigel at passage 10. Right: Representative immunofluorescent micrographs of iPSCs positive for stem cell markers: SOX2, OCT4, NANOG, and SSEA4. Nucleus is labelled with Hoechst (in blue). D) Flow cytometry analysis of pluripotency protein markers: TRA-1-60, NANOG and SSEA4. E) RT-PCR verification of the clearance of Sendai virus from the reprogrammed cells. Sendai virus vector transduced fibroblasts was used as positive control. F) Pathological analysis of a teratoma from TRNDi005-A iPSC, showing a normal ectodermal, endodermal and mesodermal differentiation.