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. 2021 Mar 31;11:635233. doi: 10.3389/fonc.2021.635233

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Copyright © 2021 Guo, Ma, Fu, Liu, Liu, Hu, Miao, Zhang, Liu, Han, You, Yang and Zheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expressions of stem cell surface markers in A549 CSCs. (A) Immunofluorescence staining of A549 cells and CSCs. A549 cells were stained for CD44 and CD133. CSCs were stained for CD133. Pictures were taken using a Nikon microscope. The bar represents a distance of 50 μm. (B) Flow cytometry analysis of CD44 expression in A549 cells and CD133 in A549 cells and CSCs. Red histograms presented cells stained with PE Rat IgG1, κ Isotype Control. Blue histograms presented cells stained with PE Anti-Human CD133. Purple histograms presented cells stained with FITC Rat IgG1, κ Isotype Control. Green histograms presented cells stained with FITC Anti-Human CD44.