Skip to main content
. 2021 Mar 31;11:651495. doi: 10.3389/fcimb.2021.651495

Figure 1.

Figure 1

General experimental schematic for pneumococcal shedding and nasal colonization with CSE treatment. (A) Schematic of the experimental schedule. From day 4-7 of life, pups were inoculated intranasally with either CSE or PBS twice a day. At day 8 of life, pups were inoculated intranasally with S. pneumoniae 6A. Pups were euthanized on day 2 or 4 p.i (day 10 or 12 of life) and the pneumococcal burden obtained from nasal lavages was evaluated. Unless otherwise noted, the experiments were performed in this schedule. (B) Nasopharyngeal pneumococcal colonization assessed by nasal lavages. Bar represents the median values and each symbol represents the CFU/ml from a single mouse. CSE group (black circle) (n = 12 in Day 2 p.i. and 26 in Day 4 p.i.) and PBS group (open square) (n = 12 in Day 2 p.i. and 22 in Day 4 p.i.). Dotted line indicates the limit of detection (666 CFU/ml). Mann-Whitney U test was used for the statistical analyses.