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. 2021 Mar 31;11:651495. doi: 10.3389/fcimb.2021.651495

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Copyright © 2021 Murakami, Kono, Nanushaj, Kaneko, Zangari, Muragaki, Weiser and Hotomi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial shedding in nasal secretions and local neutrophil influx into the nasal cavity. Quantification of daily (A) and total (all 4 days) (B) bacterial shedding. Shedding values of each day post-infection; median indicated by the bar and each symbol represents the CFU/10 taps from a single mouse on a single day (n = 13 in each group). Dotted line indicates 150 CFU/10 taps. (C) Neutrophils in the nasal lavages quantified by flow cytometry. CSE group (black circle) (n = 8) and PBS group (open square) (n = 7). Mann-Whitney U test was used for the statistical analyses.