Figure 5.

Histological analyses of the nasal cavity of pups by hematoxylin and eosin staining (A, B, G, H) and immunohistochemistry double staining of type 6A pneumococcal capsule polysaccharide and Alcian blue for mucus (C–F, I–L). CSE pre-treated group; (A–F), PBS pre-treated group; (G–L). (A, C, E, G, I, K) show low-magnification field (100×magnification. Scale bar, 100 µm) and (B, D, F, H, J, L) are high-magnification field of boxed sections (400×magnification. Scale bar, 20µm). Three mice for each group were examined for the analyses and the representative images were displayed.