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. 2021 Mar 31;12:649768. doi: 10.3389/fpls.2021.649768

FIGURE 2.

FIGURE 2

Temperature effect on TuMV-GFP infection in B. napus cultivars Drakkar and Tanto. Plants were incubated at 20°C for 8 h (night) and 24°C for 16 h (day) until two leaf stage. Half of the plants were then transferred to 20°C for 8 h (night) and 28°C for 16 h (day). Plants were allowed to adjust for 2 days before inoculation. (A) Effect of temperature on viral cell-to-cell spread in inoculated leaves. Pictures were taken at 6 days post inoculation (6 dpi) under UV light. Scale bar, 1 cm. (B) Sizes of individual local infection sites at 6 dpi. Infection foci in leaves of five plants per condition were measured (Drakkar 24°C, N = 117; Drakkar 28°C, N = 111; Tanto 24°C, N = 112; Tanto 28°C, N = 102. The higher temperature causes a significant increase in the local cell-to-cell spread of infection in both Drakkar (ANOVA, p = 4,3− 21) and Tanto (ANOVA, p = 2,5− 37). (C) Systemic spread of TuMV-GFP in Drakkar is more efficient at 28°C (orange) than at 24°C (green). Inoculated leaves of nine plants at 24°C and of nine plants at 28°C were removed after 1, 2, or 3 dpi, followed by scoring the systemic leaves for GFP fluorescence (systemic infection) at 16 dpi. A control plant from which the inoculated leaf was not removed is shown in panel (D). (D) TuMV-GFP-infected Drakkar plant showing systemic infection at 21 dpi. The picture was taken under UV light.