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. 2021 Mar 31;12:652258. doi: 10.3389/fimmu.2021.652258

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Copyright © 2021 Liu, Yao, Gong, Song, Chen, Ye, Liu, Li, Dong, Meng, Chen and Zheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Accuracy of the three tools for HLA typing at the second field or the third field resolution for different depths and read lengths. Depth evaluation at (A) the second field level; (B) the third field level. For sequence depth evaluation, alignment files of the 24 Bofuri samples were down-sampled from 700X to 10X based on the raw depths of HLA genes. (C, D) are the overall HLA typing accuracy at the second field and the third field level, respectively, while the read length decreased from 150 bp to 76 bp.